The ‘Angelo Rambelli’ Botanical Garden, inaugurated on March 24, 1991, covers approximately 6 hectares west of Viterbo, approximately 300 meters above sea level, on the right bank of the Urcionio stream, near the Bulicame spring, whose fame, linked to its thermal waters, dates back to Roman times, and likely even earlier to the Etruscan era. The area is characterized by significant annual temperature variations (min -10°C, max 40°C) and highly calcareous soil. Subsurface, at depths ranging from 4 to 8 meters, are numerous hot aquifers rich in mineral salts, especially carbonates, which have deposited over millennia, forming whitish concretions. From the Bulicame’s main spring, the water flowed through characteristic raised channels into pools where hemp was once placed to macerate. Canals and pools have now been transformed into streams and ponds that run throughout the entire facility and host aquatic ecosystems, occasionally enriched by spontaneous herbaceous vegetation. The idea of ​​creating a Botanical Garden in Viterbo originated with the first Rector of the University of Tuscia, Professor Gian Tommaso Scarascia Mugnozza. Despite the area’s chemical and physical characteristics making it unsuitable for plant growth, the challenge was taken up by Professor Angelo Rambelli, founder and first director of the Botanical Garden, after whom it is now named. It is thanks to his commitment and tenacity, and that of numerous collaborators and subsequent directors, that the Botanical Garden is today a beautiful institution, a source of cultural wealth for the entire scientific community and the city of Viterbo. The Garden is now part of the Bulicame Park, a complex spanning approximately 15 hectares, divided between the Botanical Garden, in the lower and more hospitable section, and the Bulicame sulfurous hot spring above. It works in close collaboration with the Tuscia Herbarium Museum (UTV), founded by A. Scoppola in 1996, and the Germplasm Bank, established in 2005. These three facilities are now part of the University Museum System (SMA). The Angelo Rambelli Botanical Garden plays a fundamental role in the collection and dissemination of scientific knowledge and research. It welcomes thousands of visitors annually, many of whom are students from schools of all levels and universities, and organizes and conducts exercises, internships, training courses, and events. Currently, the Botanical Garden of Viterbo, as an Interdepartmental Center of the University of Tuscia (COB), plays a fundamental role in the collection and dissemination of scientific knowledge: the Department of Environmental Sciences (DISA) of the Faculty of Mathematical, Physical and Natural Sciences, the Department of Agrobiology and Agrochemistry (DABAC), the Department of Forestry Environmental Sciences and its Resources (DISAFRI),The Department of Plant Production (DIPROV) and the Department of Plant Protection (DIPROP) of the Faculty of Agriculture. It annually hosts thousands of students of all levels who visit the facility, also conducting educational activities primarily aimed at university students: the Botanical Garden hosts practical exercises for various courses in Botany and related subjects, and in recent years has hosted numerous degree theses and internships. It also collaborates in the development of a second-level Master’s program for “Curator of Parks, Gardens, and Botanical Gardens,” courses in Mediterranean gardening, and training courses for people with disabilities. The Botanical Garden of the University of Tuscia is a founding member of the Italian Network of Germplasm Banks for the Ex Situ Conservation of Italian Spontaneous Flora (RIBES) and has joined the Working Group for Botanical Gardens of the Italian Botanical Society: a small but qualified group of members involved in various capacities in the management or use of these structures. To date, the Working Group has over 200 members and more than 50 university or local authority botanical gardens, alpine gardens, forest botanical gardens, and arboretums.